Volume 15, Issue 2 (Summer-Fall 2021)                   IJOP 2021, 15(2): 197-208 | Back to browse issues page

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Esmaeeli M, Ahmadi-Zeidabadi M, JalalKamali M, Eskandary H, Shojaei M. Inhibitory Effect of Photobiomodulation on the Proliferation Rate of the U87 Glioblastoma Cell Line. IJOP. 2021; 15 (2) :197-208
URL: http://ijop.ir/article-1-438-en.html
1- Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
2- Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. & Afzal Research Institute, Kerman, Iran.
3- Afzal Research Institute, Kerman, Iran.
Abstract:   (123 Views)

Photobiomodulation therapy (PBMT) or Low level light Therapy (LLLT), is the stimulatory effect of light on the cell behavior. It has been considered as a potential therapeutic intervention. Glioblastoma is a malignant primary brain tumor without any effective treatment. This in vitro study investigated the effect of PBMT on proliferation rate and vital activity of human glioblastoma U87 cell line. Three different wavelengths were considered: 632 nm (red light, 2.1 mW/cm2), 534 nm (green light, 1.2 mW/cm2), and 457 nm (blue light, 6.5 mW/cm2). The cell behavior was studied during a period of four hours up to 60 hours after irradiation. The irradiated cells were inspected by different assays for cell count, cell viability, cell death, and free radical production rate and were compared with the control non-irradiated ones. The results show a reduction in cell viability for all the three wavelengths. However, the effect is more pronounced for blue light. Cell death assessments, staining and flow cytometry, and NBT assay shows that blue light is not lethal, but that it reduces the free radical production rate. Temporal analysis shows that the maximum effect on cell proliferation will be observed around 48 hours after irradiation. It could be concluded that light, particularly shorter wavelengths, has an inhibitory effect on the in vitro proliferation rate of U87 cell line by affecting the energetics of the cell. The effect is stimulatory and persistent for periods comparable to cell doubling time.

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Type of Study: Research | Subject: Special
Received: 2020/12/16 | Revised: 2022/03/12 | Accepted: 2022/03/15 | Published: 2022/03/19

1. D.N. Louis, A. Perry, and G. Reifenberger, "The 2016 World Health Organization Clas-sification of Tumors of the Central Nervous System: a summary," Acta Neuropathol. (Berl.), Vol. 131, No. 6, pp. 803-820, Jun. 2016. [DOI:10.1007/s00401-016-1545-1] [PMID]
2. G. Caruso and M. Caffo, "Antisense Oligo-nucleotides in the Treatment of Cerebral Gliomas. Review of Concerning Patents," Recent Patents on CNS Drug Discovery (Discontinued), Vol. 9, pp. 2-12, 2014. [DOI:10.2174/1574889809666140307113439] [PMID]
3. J.M.A. Kuijlen, E. Bremer, J.J.A. Mooij, W.F.A. den Dunnen, and W. Helfrich, "Re-view: On TRAIL for malignant glioma ther-apy?," Neuropathol. Appl. Neurobiol. Vol. 36, No. 3, pp. 168-182, 2010. [DOI:10.1111/j.1365-2990.2010.01069.x] [PMID]
4. H.A. Fine, K.B.G. Dear, J.S. Loeffler, P.L. Mc Black, and G.P. Canellos, "Meta-analysis of radiation therapy with and with-out adjuvant chemotherapy for malignant gliomas in adults," Cancer, Vol. 71, No. 8, pp. 2585-2597, 1993. https://doi.org/10.1002/1097-0142(19930415)71:8<2585::AID-CNCR2820710825>3.0.CO;2-S [DOI:10.1002/1097-0142(19930415)71:83.0.CO;2-S]
5. I. Jovčevska, N. Kočevar, and R. Komel, "Glioma and glioblastoma - how much do we (not) know?," Mol. Clin. Oncol. Vol. 1, No. 6, pp. 935-941, 2013. [DOI:10.3892/mco.2013.172] [PMID] [PMCID]
6. M.R. Hamblin and Y.Y. Huang, Handbook of photomedicine, Taylor & Francis, 2013. [DOI:10.1201/b15582]
7. J. Robijns, S. Censabella, P. Bulens, A. Maes, and J. Mebis, "The use of low-level light therapy in supportive care for patients with breast cancer: review of the literature," Lasers Med. Sci. Vol. 32, No. 1, pp. 229-242, 2017. [DOI:10.1007/s10103-016-2056-y] [PMID]
8. C.R. Simpson, M. Kohl, M. Essenpreis, and M. Cope, "Near-infrared optical properties of ex vivo human skin and subcutaneous tissues measured using the Monte Carlo in-version technique," Phys. Med. Biol. Vol. 43, No. 9, pp. 2465-2478, 1998. [DOI:10.1088/0031-9155/43/9/003] [PMID]
9. K.C. Smith, "Laser (and LED) Therapy Is Phototherapy," Photomed. Laser Surg. Vol. 23, No. 1, pp. 78-80, 2005. [DOI:10.1089/pho.2005.23.78] [PMID]
10. S. Passarella and T. Karu, "Absorption of monochromatic and narrow band radiation in the visible and near IR by both mito-chondrial and non-mitochondrial photoac-ceptors results in photobiomodulation," J. Photochem. Photobiol. B, Vol. 140, pp. 344-358, 2014. [DOI:10.1016/j.jphotobiol.2014.07.021] [PMID]
11. R.T. Chow, M.I. Johnson, R.A. Lopes-Martins, and J.M. Bjordal, "Efficacy of low-level laser therapy in the management of neck pain: a systematic review and meta-analysis of randomised placebo or active-treatment controlled trials," The Lancet, Vol. 374, No. 9705, pp. 1897-1908, 2009. [DOI:10.1016/S0140-6736(09)61522-1]
12. T. Fushimi, S. Inui, T. Nakajima, M. Ogasawara, K. Hosokawa, and S. Itami, "Green light emitting diodes accelerate wound healing: Characterization of the ef-fect and its molecular basis in vitro and in vivo," Wound Repair Regen. Vol. 20, No. 2, pp. 226-235, 2012. [DOI:10.1111/j.1524-475X.2012.00771.x] [PMID]
13. S. Dehghani-Soltani, M. Shojaei, M. Jalalkamali, A. Babaee, and S.N. Nematol-lahi-mahani, "Effects of light emitting diode irradiation on neural differentiation of hu-man umbilical cord-derived mesenchymal cells," Sci. Rep. Vol. 7, No. 1, 2017. [DOI:10.1038/s41598-017-10655-w] [PMID] [PMCID]
14. A.N. Pereira, C. de P. Eduardo, E. Matson, and M.M. Marques, "Effect of low-power laser irradiation on cell growth and procol-lagen synthesis of cultured fibroblasts," La-sers Surg. Med. Vol. 31, No. 4, pp. 263-267, 2002. [DOI:10.1002/lsm.10107] [PMID]
15. D.H. Hawkins and H. Abrahamse, "The role of laser fluence in cell viability, prolif-eration, and membrane integrity of wound-ed human skin fibroblasts following heli-um-neon laser irradiation," Lasers Surg. Med. Vol. 38, No. 1, pp. 74-83, 2006. [DOI:10.1002/lsm.20271] [PMID]
16. F.Y. Ang, Y. Fukuzaki, B. Yamanoha, and S. Kogure, "Immunocytochemical studies on the effect of 405-nm low-power laser ir-radiation on human-derived A-172 glioblas-toma cells," Lasers Med. Sci. Vol. 27, No. 5, pp. 935-942, 2012. [DOI:10.1007/s10103-011-1009-8] [PMID]
17. S.P. Longdon, Cancer Cell Culture - Meth-ods and Protocols, Vol. 2nd Edition. Hu-mana Press, 2004.
18. Y.A. Hannun and R.-M. Boustany, Apopto-sis in Neurobiology, CRC press, 1998. [DOI:10.1201/9780367801946]
19. A.S.H. Ong and L. Packer, Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications, 1992. [DOI:10.1007/978-3-0348-7432-8]
20. R. Sroka, M. Schaffer, C. Fuchs, T. Pongratz, U. Schrader Reichard, M. Busch, P.M. Schaffer, E. Dühmke, and R. Baum-gartner, "Effects on the mitosis of normal and tumor cells induced by light treatment of different wavelengths," Lasers Surg. Med. Vol. 25, No. 3, pp. 263-271, 1999. https://doi.org/10.1002/(SICI)1096-9101(1999)25:3<263::AID-LSM11>3.0.CO;2-T [DOI:10.1002/(SICI)1096-9101(1999)25:33.0.CO;2-T]
21. H. Murayama, K. Sadakane, B. Yamanoha, and S. Kogure, "Low power 808 nm laser irradiation inhibits cell proliferation of a human-derived glioblastoma cell line in vitro," Lasers Med. Sci. Vol. 27, No. 1, pp. 87-93, 2012. [DOI:10.1007/s10103-011-0924-z] [PMID]
22. Y. Fukuzaki, F. Yee Ang, B. Yamanoha, and S. Kogure, "Effects of 532 nm Low power Laser Irradiation on Cell Prolifera-tion of Human-derived Glioblastoma," Nippon Laser Igakkaishi, Vol. 32, No. 4, pp. 382-388, 2012. [DOI:10.2530/jslsm.32.382]
23. R. Zein, W. Selting, and M.R. Hamblin, "Review of light parameters and photobio-modulation efficacy: dive into complexity," J. Biomed. Opt. Vol. 23, No. 12, p. 1, 2018. [DOI:10.1117/1.JBO.23.12.120901] [PMID] [PMCID]
24. H. Serrage, V. Heiskanen, W.M. Palin, P.R. Cooper, M.R. Milward, M. Hadis, and M.R. Hamblin, "Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light," Photochem. Photobi-ol. Sci. Vol. 18, No. 8, pp. 1877-1909, 2019. [DOI:10.1039/C9PP00089E] [PMID] [PMCID]

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