International Journal of

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The identification and concentration of heavy metals, which may be so harmful for the body, is determined by the method of calibration-free laser-induced breakdown spectroscopy using a special strategy. First, the plasma temperature is obtained using the Boltzmann plot. Then, a line with an inappreciable self-absorption is considered for each element as the reference. The modified intensities of other lines of the element are calculated through their self-absorbed intensities in terms of the reference intensity. The plasma temperature is again computed by line pair ratio method. This procedure is carried out by an iterative algorithm until the self-absorption coefficient of selected lines converges on one. In the last step, the corrected temperature is evaluated by the Boltzmann plot drawn using true (non self-absorbed) line intensities of each element. The concentration of the elements is finally determined by the corrected temperature and intensities. The results indicate that the accuracy of this method in determining the concentrations is significantly better than the normal way.

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Simultaneous laser induced- breakdown spectroscopy (LIBS) and acoustic response techniques as well as Laser induced fluorescence (LIF) are applied to investigate the abnormal lymph tissues due to Hodgkin disease. The spectral shift in the emissive fluorescence of the cancerous tissues has been observed respect to the normal ones. Regarding LIBS, the concentrations of Ca and Na trace elements have been identified to be higher in the cancerous samples. In addition, the acoustic response of cancerous tissues has been elevated against healthy ones. The distinct differences in the spectra are taken into account for early and the rapid identification and diagnosis.