Simultaneous laser induced- breakdown spectroscopy (LIBS) and acoustic response techniques as well as Laser induced fluorescence (LIF) are applied to investigate the abnormal lymph tissues due to Hodgkin disease. The spectral shift in the emissive fluorescence of the cancerous tissues has been observed respect to the normal ones. Regarding LIBS, the concentrations of Ca and Na trace elements have been identified to be higher in the cancerous samples. In addition, the acoustic response of cancerous tissues has been elevated against healthy ones. The distinct differences in the spectra are taken into account for early and the rapid identification and diagnosis.