The average velocity of water flow has been simultaneously measured with fluorescent and speckle imaging methods. The measured velocities with two methods are in good agreement with each other and it confirms that the speckle imaging method can be used as a confident method to measure the velocity of water flow in a dry leaf. Also the velocity of water flow through thick and thin xylems of a leaf can be measured with speckle imaging method and the ratio of thick xylem’s radius to thin xylem’s radius can be estimated with this method. Also the ratio was measured by monitoring the cross section of the leaf. The two measurements were in agreement.